23 Jan 2018

Genome Res. published online January 9, 2018

Jun Ding Bruce Aronow Naftali Kaminski Joseph Kitzmiller Jeffrey Whitsett and Ziv Bar-Joseph

Reconstructing differentiation networks and their regulation from time series single cell expression data

"Generating detailed and accurate organogenesis models using single cell RNA-seq data remains a major challenge. Current methods have relied primarily on the assumption that descendant cells are similar to their parents in terms of gene expression levels. These assumptions do not always hold for in-vivo studies which often include infrequently sampled, un-synchronized and diverse cell populations. Thus, additional information may be needed to determine the correct ordering and branching of progenitor cells and the set of transcription factors (TFs) that are active during advancing stages of organogenesis. To enable such modeling we have developed a method that learns a probabilistic model which integrates expression similarity with regulatory information to reconstruct the dynamic developmental cell trajectories. When applied to mouse lung  developmental data the method accurately distinguished dierent cell types and lineages. Existing and new experimental data validated the ability of the method to identify key regulators of cell fate."

Please visit the Supplemental Website for more informationhttp://www.cs.cmu.edu/~jund/scdiff/index.html