3D MMVR

LungMAP now offers a tool to experience actual data in a completely unique way. An integrated IO visualization tool called 3D MicroCT/Microscopy Volume Renderer --- 3D MMVR ---- does direct volume rendering using ray casting, a true volumetric representation of the data. Ray casting preserves detail and allows for the quick adjustment of opacities. You can look into structures and see what happens inside. We are very excited to offer this unique experience to the lung community.

Here is a manual describing usage of the tool and a video tutorial is available below.

Quick Start Guide to help you get started

 

 

The 3D viewer will open into this dataset for you to explore:

  • C57Bl6 Adult mouse lung, treated with 4 doses of intratracheal annexin V at a normal human physiological level of 7.5 ng per dose demonstrates microCT appearance indistinguishable from controls. Lungs perfusion fixed at 25 cm H2O airway pressure by cardiac puncture, scanned at a nominal resolution of 6 um using a Bruker Skyscan microCT, reconstructed using the proprietary Bruker software based on the Feldkamp algorithm. This data is in DICOM File structure.

Other datasets are available for viewing. Choose the "Load Volume" top button in left side panel of 3DMMVR.

  • [P07C006] C57Bl6 P07 Mouse Lung, perfusion fixed and dried at 20 cm H2O airway pressure and 30 cm H2O vascular pressure using a modified Hoffman protocol, scanned at 2.7 micron nominal resolution using a Bruker 1172 Skyscan microCT, reconstructed using the proprietary Bruker software based on the Feldkamp algorithm. Mouse serial number 0006; this data is in DICOM file structure.
  • [P21C002] C57Bl6 P21 Mouse Lung, perfusion fixed and dried at 20 cm H2O airway pressure and 30 cm H2O vascular pressure using a modified Hoffman protocol, scanned at 2.7 micron nominal resolution using a Bruker 1172 Skyscan microCT, reconstructed using the proprietary Bruker software based on the Feldkamp algorithm. This data is in DICOM File structure
  • [P28C005] C57Bl6 P28 Mouse Lung, perfusion fixed and dried at 20 cm H2O airway pressure and 30 cm H2O vascular pressure using a modified Hoffman protocol, scanned at 2.7 micron nominal resolution using a Bruker 1172 Skyscan microCT, reconstructed using the proprietary Bruker software based on the Feldkamp algorithm. This data is in DICOM File structure

 

NOW AVAILABLE: 2 HUMAN LUNG DATASETS!

About the samples: The lungs arrived with trachea occluded by tie or staple. The trachea was opened and intubated with an endotracheal tube (ETT) which was then held in place with a plastic zip tie. The ETT was attached to a T=piece resuscitator (Neopuff) and inflated with air (21% oxygen) to 25 cm H20 pressure. The lungs were kept cold and moist in a plastic bag on ice. A CT scan was performed at this steady distending pressure, with the lungs lying supine on a bed of cotton in as close to anatomical position as could be maintained.

Datasets include a 5 day old male (HTCD004) and a 9 year old male (HTCD016) human lung sample. There is no known lung disease in either donor. Other details about the donors can be provided upon request. Connect to MMVR to explore these human samples.

LungMAP 3DMMVR

If you do not have a remote desktop client installed, click here to download the appropriate one for your computer. Then click on the green arrow icon to download the remote desktop configuration file needed for 3DMMVR. Next, click on the downloaded file to start the session.

You will be asked for this password:
lungmaps3cr3t! (click to copy)